منابع مشابه
Radioimmunoassay of factor V in human plasma and platelets.
Homogeneous, single-chain human factor V was used to develop a double antibody competition radioimmunoassay to measure factor V concentrations in plasma and platelets. Standard curves were constructed that allow for the detection of as little as 20 ng factor V/ml of plasma. Normal factor V concentrations range from 4 to 14 micrograms/ml of plasma with an average value of 7.0 +/- 2.0 micrograms/...
متن کاملTriiodothyronine radioimmunoassay.
Highly specific antisera to triiodothyronine (T(3)) were prepared by immunization of rabbits with T(3)-bovine serum albumin conjugates. Antisera with T(3) binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T(3-) (125)I to anti-T(3) serum was found to vary considerably. l-T(3), d-T(3) and several triiodoanalogues were potent ...
متن کاملPhytochrome radioimmunoassay.
A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay and requires less than 1 microliter of crude extract from dark-grown plants for quantitation of phytochrome. Measurement of phytochrome in crude extracts by radioimmunoassay gives values about 25% gre...
متن کاملRapid radioimmunoassay of triiodothyronine.
The method used in this report is based on the immunological production of a specific antisera to tnlodothyronine (T3) in rabbits. Conducted at 4 #{176}C, the radioimmunoassay requires three to five days to complete. Hoping to speed this assay, we investigated the influence of time, temperature, and pH. Incubation at 37 #{176}C and pH 8.0 for 30 mm did not deleteriously affect either precision,...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1971
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.20.11_638